Generator
Part:BBa_K325219:Design
Designed by: Bill Collins and Emily Knott Group: iGEM10_Cambridge (2010-09-15)
Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 3278 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 2169 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 2118
Illegal AgeI site found at 3820 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Illegal SapI.rc site found at 1550
Illegal SapI.rc site found at 3334
Design Notes
Biobrick assembly
Source
Biobrick assembly from its two subparts