Generator

Part:BBa_K325219:Design

Designed by: Bill Collins and Emily Knott   Group: iGEM10_Cambridge   (2010-09-15)

Red Firefly Luciferase and LRE (under pBAD)
L. Cruciata
(E. coli optimised)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334


Design Notes

Biobrick assembly


Source

Biobrick assembly from its two subparts

References